Mitochondria in pancreatic cancer cells colour-coded to show how they move over time. Mitochondria in pancreatic cancer cells colour-coded to show how they move over time. Credit: V. Papalazarou and N.R. Paul, Cancer Research UK Beatson Institute
Waiting in the starting blocks, sprinters’ muscles are about to need to produce a lot of energy. And quickly. For short bursts of energy like this, the body can’t rely on its usual methods of production.
Instead, it relies on a store of a molecule called creatine phosphate, which can be rapidly turned into creatine in a reaction that releases a lot of energy, acting like a turbo boost.
As the pistol goes off, stored creatine phosphate is converted, the sprinter’s muscles contract, and they’re on their way to the finish line.
Surprisingly, this cascade of chemicals is also used by pancreatic cancers when they begin to spread.
Professor Laura Machesky, an expert in cancer spread at the Cancer Research UK Beatson Institute, has been interested in how pancreatic cancer spreads for a number of years.
Pancreatic cancers are known for being particularly aggressive and tend to spread to other parts of the body in a process called metastasis. They’re also notorious for being very rigid (stiff) tumours.
“We wanted to know if pancreatic cancers’ signature stiffness could be making it more aggressive,” says Machesky.
But before they could get out the starting block, they needed to be able to recreate the race conditions in the lab. And for that, they needed an engineer.
Professor Manuel Salmeron-Sanchez, an expert in biomedical engineering at the University of Glasgow, leads a team that creates various materials to study tissue repair and regeneration.
He works on ‘hydrogels’ – gels made of different chains of molecules (polymers) suspended in water. Because hydrogels have a degree of flexibility and act like body tissue, they’re useful for making things like contact lenses. But they’re handy in the lab too.
Salmeron-Sanchez’s lab were tasked with designing materials that could recreate pancreatic tumours of different stiffnesses.
“Collaboration between our labs was essential to this research,” says Machesky. “Teaming up with labs of different disciplines allows you to see things in a different way, and none of us would have been able to produce these results on our own.”
With the materials in place, the race could finally begin.
Publishing their work in Nature Metabolism, the team found that pancreatic cancer cells grown on a stiff tumour environment became more mobile.
This happened with cancer cells that had identical DNA, which revealed that the aggressive nature of the cancer wasn’t entirely hard wired – the physical surroundings of the cell played a big role as well.
Interestingly, cells grown in a stiffer environment also created and used more energy. Which led to the team’s next question – where is this extra energy coming from?
“This is an interesting question as pancreatic tumours are generally thought to have poor access to oxygen and nutrients, which are essential fuel for cancer cells,” says Dr Oliver Maddocks, an expert in cancer metabolism at the University of Glasgow Institute of Cancer Sciences. “However, previous research has shown that cancer cells can be quite flexible when it comes to finding sources of energy,”
Maddocks’ lab specialises in cancer metabolism, which allowed the teams to look at the energy processes taking place within the cells and helped piece together what was really going on.
Making a sprint
Cells that need to move a lot, like immune cells that patrol the body looking for invaders, move their internal powerpacks – structures called mitochondria – to the edge of the cell in the direction they want to move.
But another common way for cells to produce energy is through the stored creatine phosphate we use in sprinting.
And it turns out that the pancreatic cancer cells grown in stiffer environments also had higher levels of creatine and an enzyme called creatine kinase B-type (CKB), which turns creatine phosphate into creatine.
This result was promising for the team’s theory, so they then tested whether removing CKB from a cell affected its ability to migrate. And encouragingly it did – cancer cells that lacked CKB couldn’t move in the same way as regular cancer cells.
This showed for the first time that CKB is an important link between cell migration, metabolism, and the physical properties of pancreatic cancer’s environment.
Based on these links, it’s possible that targeting CKB could be an option for preventing metastasis in pancreatic cancer.
This theory was backed up by some initial testing in mice, where tumours with CKB removed or blocked spread less from the spleen to the liver. The team are hoping to investigate this further, along with experimenting with more complicated materials that are even closer to the real tumour environment.
And Machesky is excited about what comes next from this collaboration. “We went into this research with no preconceptions of what we might find, but ended up with some exciting results that we’re looking forward to pursuing even further.”
Harry Jenkins is a science media officer at Cancer Research UK
Papalazarou, V. et al. (2020) The creatine–phosphagen system is mechanoresponsive in pancreatic adenocarcinoma and fuels invasion and metastasis. Nature Medicine. DOI: 10.1038/s42255-019-0159-z
Elizabeth Lees June 27, 2020
Lost my Mum to this cancer 30 years ago and progress has been slow. However, pleased to read that Research is in progress.
Jacky Glendinning June 24, 2020
Fascinating. Thanks for sharing.
Sian bevan June 24, 2020
That’s so interesting, makes me feel hopeful for the future that one day there might be a cure to this particularly horrible cancer. Keep up this wonderful work. Thankyou
Su Clarke June 22, 2020
That’s amazing and really interesting. Thank you for sharing this with us.
Joanne June 21, 2020
It’s good to hear research is being done into pancreatic cancer, I lost both my parents to this cancer. Let’s hope that one day people will be able to survive it.
Linda Thomson June 21, 2020
Pleased to hear of the research into Pancreatic Cancer having lost my father to this dreadful and aggressive Cancer aged 59 40 years ago and then my sister aged 59 in 2017 They were both so brave. I hope that one day there will be a cure not only for this one but others too
Murray Hutchison June 20, 2020
Better news. Too little progress in 30 years on pancreatic cancer. Glad to see a focus in Glasgow. I wonder if that is because Scotland has a higher incidence of this cancer. Personally I have lost 3 friends to it and know personally two others have been able to have surgery to remove their tumour. I would love to see survival rates significantly improved and earlier diagnostic methods. I also wonder if it is increasing in incidence.
Dorothy Lopez-Ferreiro June 20, 2020
I am so glad that progress is being made in combating pancreatic cancer and would like the scientists involved to know how much their painstaking research is appreciated.
Debbie Suckling June 20, 2020
This is an exciting development. A light for the future for this particularly aggressive cancer. Some hope means everything. Well done
Angela Mitchell June 18, 2020
Some positive news about pancreatic cancer. This is a dreadful cancer that was hard to detect with very little survival rate 25 years ago. Truly heartbreaking watching a loved one pass to this cancer. Really pleased that research is being done. Interesting and encouraging. Keep up the good work and thank you to all.
H Nevill June 18, 2020
Really interesting. Thank you for sharing.
Carole Pearse June 18, 2020
Pancreatic cancer prognosis is usually dire, unless it can be detected at an extremely early stage.I feel research into this should be paramount. Is there any research out there investigating this?
Victoria Norledge June 18, 2020
It’s so good to hear that research is being done into pancreatic cancer. I wish we didn’t know what a death sentence that is for a family member. To read that it’s being looked into with such detailed care brings hope that this will also one day be a cancer you can survive. Thank you.